The spectramax m3, m4, m5 and m5e multimode microplate readers are a modular, upgradeable dualmonochromator microplate reader platform offering a wide range of high performance multimode capabilities ideal for life science research and drug discovery screening. Application note measuring luciferase expression using. The upgradeable spectramax m5 multimode microplate reader comes with the standard five modes of detection with the upgrade option to add htrf at a later time. The molecular devices spectramax m3 multimode microplate reader covers a wide range of applications without the need to change filters. The spectramax id3 and id5 multimode microplate readers measure absorbance, fluorescence, and luminescence. Molecular devices spectramax 340pc microplate reader. Spectramax m5 microplate reader from molecular devices llc. This revolution in modularity makes nocompromise detection the norm. Detection modalities include absorbance uvvis abs and fluorescence intensity fi and have optical performance comparable to a topoftheline dedicated spectrofluorometer or spectrophotometer. Spectramax microplate spectrophotometer operators manual. The spectramax m3 multimode microplate reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. This easy to use multimode plate reader is designed to handle the work flow of multiple research projects.
All spectramax m series multimode microplate readers measure uv and visible absorbance, and fluorescence intensity measurements on microplates and cuvettes as standard. Spectramax m3, m4, m5, and m5 e multimode microplate. Fluorescence plate reader molecular device, spectramax geminixs. With optimized reagents and the industryleading data acquisition and analysis tool, softmax pro 7 software, the spectramax to perfectly match your needs. In these mutants, intermediate metabolites of adenine biosynthesis pathway are accumulated. In this guide, well show you the steps to get started with the new pdf features included with microsoft edge, introduced in the windows 10 fall creators update. In addition, the id5 reader measures trf and fp and can be expanded to include trfret, htrf, bret, dual luciferase reporter assays with injectors, and western blot detection.
Spectramax i3x multimode detection platform, molecular. This dual monochromatic, multi mode microplate reader delivers three modes of detection, uv vis absorbance reading, fluorescence and luminescence, and a broad spectrum of formats from cuvettes to 6, 12, 24, 48, 96, and 384. Robust, highvalue microplate readers can run samples based on predefined protocols and standard filter modes cover the entire visible range for a variety of assays. Setup guide on the molecular devices spectramax m5m5e. Description the onboard microprocessor calculates and reports the absorbance, % transmittance, andor fluorescence for each well of a microplate or from the cuvette port. All books are in clear copy here, and all files are secure so dont worry about it. Using the molecular devices test plate to calibrate the. Using the molecular devices test plate to calibrate the spectramax reader. Spectramax plus 384 microplate spectrophotometer operators manual iii this warranty only covers defects arising under normal usage and does not cover malfunctions of failures resulting from misuse, abuse, neglect, alteration, modification, or repairs by other than an authorized molecular devices corporation service facility. Spectramax plus 384 microplate reader manual conquer scientific. Spectramax plate reader eastern virginia medical school.
The spectramax m3 microplate reader is the standard for uvvisible multimode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6, 12, 24, 48, 96, and 384well microplates. Molecular devices spectramax 250 microplate reader. Spectramax m3 microplate reader from molecular devices llc description the spectramax m3 multimode microplate reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Dec 03, 20 the spectramax minimax 300 imaging cytometer enables cell visualization and cellbased analysis on the spectramax i3 multimode microplate reader.
The spectramax 190 reader is the tool for dna analysis. With optimized reagents, option to operate the readers. Spectramax m5e multimode microplate reader youtube. Spectramax i3 multimode detection platform youtube. The hdl cholesterol content was measured by a colorimetric assay thermo electron, inc. Molecular devices spectramax id3 multimode microplate reader special introductory offer. The spectramax m series multimode microplate readers measure uv and visible absorbance, fluorescence, luminescence, fluorescence polarization, trf and htrf. Robust, highvalue microplate readers that can run samples based on predefined protocols and standard filter modes cover the. The spectramax m2 and m2e readers provide two detection modes in one platform, and offer the the added functionality of bottomread fluorescence for cellbased assays m2e. This tunable visible absorbance reader is for 96 and 384well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340850 nm, tunable in 1 nm.
Spectramax id3 multimode microplate reader molecular. The spectramax id5 multimode microplate reader is the complete laboratory solution to help you increase your research capabilities and comes with builtin absorbance, fluorescence, luminescence, timeresolved fluorescence trf, and tunable fluorescence polarization fp read. This dual monochromatic, multi mode microplate reader delivers three modes of detection, uv vis absorbance reading, fluorescence and luminescence, and a broad spectrum of formats from cuvettes to 6, 12, 24, 48, 96, and 384well microplates. Spectramax m3 reader spectramax m4 reader spectramax m5 reader spectramax m5e reader detection modes. Robust, highvalue microplate readers that can run samples based on predefined protocols and standard filter modes cover the entire visible range for a variety of assays. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Spectramax glo steadyluc reporter assay kit workflow. Molecular devices spectramax m2 multimode microplate reader. Molecular devices spectramax 250 microplate reader marshall. For more detailed information and technical support of molecular devices instruments or software.
The spectramax i3 x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and. Spectramax id3 and id5 multimode microplate readers. Spectramax m3, m4, m5, and m5e multimode microplate reader. Spectramax m3 multimode microplate reader from molecular. Microplate readers, multimode, spectramax m series vwr. With optimized reagents and the industryleading data acquisition and analysis tool softmax pro 7 software, the spectramax id3 allows customization of workflow to. The pathcheck sensor can extend the instrument linearity by a factor of three or more. Operators manual molecular devices spectramax 190 absorbance plate reader. Spectramax 190 microplate spectrophotometer operators manual vii conventions used in this manual the names of keys that appear on the spectramax 190 control panel are shown in boxed helvetica type. The spectramax id3 multimode microplate reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The molecular devices spectramax 340pc microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Paraoxonase activity assay ann arbor, mich and the spectramax m5 plate reader cited by 161 related articles all 11 versions pdf academia. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. Spectramax m3, m4, m5, and m5e multimode microplate.
This reader does uv and visible absorbance, and fluorescence intensity measurements on microplates and cuvettes, standard. Spectramax m3 multimode microplate reader, molecular devices. Molecular devices just launched spectramax id3 multimode microplate reader is the cornerstone of complete laboratory solution helping to expand the boundaries of research. Molecular devices spectramax id5 multimode microplate reader.
Software for the test plate must be installed on the control computer before the plate can be used. Detection modes include absorbance uvvis abs and fluorescence intensity fi and have. Spectramax m series multimode microplate readers molecular. The ability to detect 16 ngwell of dna and to quantitate 50 ng of dna provides the same sensitivity as a spectrophotometer. The spectramax i3x microplate reader from molecular devices evolves with your future research needs and offers an unlimited breadth of applications the spectramax i3x multimode microplate reader measures spectralbased absorbance, fluorescence, and luminescence with the added functionality. These robust readers have been placed in labs from antarctica to the. Molecular devices spectramax id3 multimode microplate reader. I used the pdf reader that comes with the surface before that but its slow to add pen markings and you theres not a lot of customizing options.
Each sample has a discrete sample and reference beam so that each well is measured directly. Read online spectramax m3, m4, m5, and m5 e multimode microplate readers book pdf free download link book now. The spectramax m3 multimode microplate reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Spectramax id3 multimode microplate reader the spectramax id3 multimode microplate reader is the cornerstone of a. The molecular devices spectramax m5m5e microplate reader was tested for compatibility with life technologies geneblazer assays. Fluorescence plate reader molecular device, spectramax geminixs 1 turn on the instrument spectramax geminixs and allow the instrument for selfcalibration and initialization. The spectramax i3x multimode detection platform measures spectralbased absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Molecular devices spectramax plus384 microplate reader manual. Future ready the spectramax i3 x multimode microplate reader measures spectralbased absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Spectramax m3, m4, m5, and m5 e multimode microplate readers.
The cuvette chamber spectramax plus 384 only figure 1. For very concentrated samples, use a minimum volume. The eightchannel system consists of 8 sample beams and 8 reference beams. The spectramax i3x platform from molecular devices is a multimode microplate reader system that evolves with your future needs and offers an unlimited breadth of application possibilities. I platebased assay for studying how fungal volatile compounds vcs affect plant growth and development and the identification of. Softmax pro software has been integrated by many leading robotics and. These settings are also valid for the spectramax m3m4, flexstation 3 multimode. Feb 09, 2011 the spectramax m5e multimode microplate reader from molecular devices delivers singlemode reader performance in one multimode reader package. The spectramax m5 multimode microplate reader is owned by the nadler lab and available to all investigators through user fees. Spectramax m series multimode microplate readers 7 the spectradrop microvolume microplate offers the ability to use as little as 2l samples with 24 or 64well plates.
All m series readers accept cuvettes and 6, 12, 24, 48, 96, and 384well microplates. The broad array of spectramax i3x detection cartridges enables the user. Spectramax m2 is a dualmonochromator, multidetection microplate reader with a dualmode cuvette port and 6384 microplate reading capability. Download spectramax m3, m4, m5, and m5e multimode microplate. Read online spectramax m3, m4, m5, and m5e multimode microplate. Uvvisible absorbance abs fluorescence intensity fi glow luminescence lumi the. These settings are also valid for the spectramax m3. Spectramax m5 multimode microplate reader from molecular. For fluorescence intensity assays, users can select from top or bottomread modes for improved sensitivity in solution and cellbased assays. The adobe one is very buggy and slow with handwriting. The molecular devices spectramax 250 microplate reader has an optical design that mimics a dualbeam spectrophotometer. The spectramax i3x multimode detection platform utilizes a patentpending design that allows for realtime system configuration by the user in less than two minutes. Spectramax m series multimode microplate readers biothing. Molecular devices spectramax id5 multimode microplate.
Spectramax id3 multimode microplate reader the spectramax id3 multimode microplate reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Spectramax m2m2e user guide university of michigan. Add reagent into cell cover and mix luminescence measurement data analysis spectramax i3i3x multimode microplate reader softmax pro software 0 250000 500000 750000 000 00 0 300000 4 0 500000 fgwe l luciferase r l u r2 0. Spectramax m3 microplate reader from molecular devices llc. Download spectramax m3, m4, m5, and m5 e multimode microplate readers book pdf free download link or read online here in pdf.
The molecular devices spectramax m2 is a dualmonochromator, multidetection microplate reader with a dualmode cuvette port and 6384 microplate reading capability. Compatible automation solutions for spectramax readers. Description the onboard microprocessor calculates and reports the absorbance, % transmittance, andor fluorescence for each well of a microplate or from the. The following document is intended to demonstrate setup of this instrument. The spectramax m5 multimode microplate reader delivers single mode reader performance in one multimode reader package. The spectramax m3, m4, m5, and m5 e microplate readers are a series of dualmonochromator, multidetection, multimode instruments with a triplemode cuvette port and 6well to 384well microplate reading capability. If the plate is new, or has been recertified since the last calibration, the new absorbance values must be entered in the calibration software. Molecular devices spectramax id3 multimode microplate. How to use microsoft edge as a pdf reader in the windows. Molecular devices spectramax m3 multi mode microplate reader. Spectramax and versamax plate readers operators manual 01120126 rev a 5 1.
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